Ant and Dec, both 42, have presented alongside one another since meeting 30 years ago.
However, Dec will host the ITV show Britain’s Got Talent without his TV sidekick for the upcoming live episodes for the first time.
Ant took a step back from his current TV commitments in order to seek rehab treatment last month, after the star was charged with drink-driving.
Ahead of the live show, ITV has also announced that the live episodes will be broadcast from London’s Hammersmith Apollo.
A statement released by the broadcaster said: “Dec will be hosting the live shows which for the first time ever are coming from the Hammersmith Apollo while Ant steps down from his TV commitments for now.
“We send Ant all our love. And we know that Dec will do a brilliant job.”
Dec took to the stage without his best friend and TV partner for the first time last month, to present the final two episodes in this series of Ant and Dec’s Saturday Night Takeaway.
The ITV veteran was supported by Saturday Night Takeaway presenters Scarlett Moffatt and Stephen Mulhern as he hosted the live entertainment show.
Meanwhile, Britain’s Got Talent judge David Walliams spoke out about Ant’s arrest as he expressed his sympathy for the troubled star.
Speaking about Ant and Dec during an interview with the Radio Times, the comedian said: “They are incredibly relatable and recognisable, and the hard thing about when you’re well-known is that when things go awry in your life, it gets played out in public.”
It came as The Daily Star Sunday reported the the pair may not appear in the next series of the Australian-based show, which is expected to return in November.
According to the publication, a show source said: “There’s a very real possibility that Ant won’t be available to present the next series of the jungle.
“While Ant and Dec are a big part of the show, they aren’t bigger than the show. They are confident it would still work without them.
“If their return is going to be a problem, producers aren’t afraid to axe them from the format and get new presenters in.”
Britain’s Got Talent’ returns this Saturday at 8pm on ITV.